치주인대세포의 증식 및 세포활성에 미치는 1.25-(OH)2D3의 영향에 관한 연구
THE EFFECT OF 1.25-(OH)2D3 ON THE PROLIFERATION AND ALKALINE PHOSPHATASE ACTIVITY OF HUMAN PERIODONTAL LIGAMENT CELLS
국윤아, 김형룡, 김상철,
소속 상세정보
국윤아 ( ) - 원광대학교 치과대학 교정학교실
김형룡 ( ) - 원광대학교 치과대학 교정학교실
김상철 ( ) - 원광대학교 치과대학 치과약리학교실
KMID : 0361919950250030333
Abstract
The hormonally active vitamin D metabolite, 1,25-dihydrouy vitamin D3 [1.25{OH)2D3J is one of the several humoral factors that may regulate osteoblast differentiation. The purpose of this study was to evaluate the effects of L25{OH) % on the PDL cells. Human PDL cells were prepared from the first premolar tooth extracted for the orthodontic treatment and they were incubated in the environment of 37°C, 5% C02 and 95% humidity.
[3M -tbymidine incorporation as a measure of proliferation potential and alkaline phosphatase activity were evaluated at 10n! , 100nM 1,25-(OH)2Da
The observed results were as follows.
1. 1,25.(OH)2D3 was significantly enhanced [3H]-thymidine incorporation at 1OOnI But did not affect by lOnAC
2. 1;2540H)21)3 was significantly increased alkaline phosphatase activity at 1 day and 6 days in a dose-dependent manner.
키워드
치주인대세포;Alkaline Phosphatase;1.25-(OH)_(2)D_(3);DNA합성;Periodontal Ligament;Alkaline Phosphatase;1.25-(OH)_(2)D_(3);Synthesis
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